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Small bowel tissue high-energy phosphate alterations during hypothermic storage using different protecting solutions.

H Burgmann·H Reckendorfer·M Sperlich·P G Spieckermann
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Abstract

Since posttransplant function is closely related to energy status after the storage period, the aim of our study was to determine high-energy phosphates in rat small bowels after 21 h of simple ischemic cold storage using different storage solutions. We compared Eurocollins-solution, Bretschneiders-HTK-solution and a modified University of Wisconsin-solution. After flushing the small bowel both intravascularly and intraluminally with the protecting solution, segments of rat small bowel were stored in the same solution at 4 degrees C for 21 h. Determination of high-energy phosphates by high-performance liquid chromatography were done at indicated times. We found that small bowel stored in Eurocollins-solution produced the best graft viability after 21 h of cold storage by maintaining a sufficient level of high-energy phosphates. The high ATP levels were produced by the pronounced anaerobic glycolysis which was facilitated by the high concentration of glucose in the Eurocollins-solution.

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AdenosineAdenosine DiphosphateAdenosine TriphosphateAllopurinolAnimalsCryopreservationGlucoseGlutathioneHypertonic SolutionsInsulinIntestine, SmallMaleMannitolOrgan PreservationOrgan Preservation SolutionsPotassium ChlorideProcaineRaffinoseRatsRats, Inbred StrainsSolutionsComparative StudyJournal Article

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H Burgmann, H Reckendorfer, M Sperlich, P G Spieckermann. 1992. Small bowel tissue high-energy phosphate alterations during hypothermic storage using different protecting solutions.. https://doi.org/10.1159/000129192

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