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PubMed · 15839641

Optically addressed droplet-based protein assay.

Kenneth T Kotz·Yu Gu·Gregory W Faris
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Abstract

We demonstrate a new method for performing protein assays with very small volumes ( approximately 1.7 muL to 14 pL). Using a laser to modify local surface energy, we manipulate, fuse, and mix droplets containing horseradish peroxidase and its substrates. A detection limit of approximately 30 attomoles of reacting enzyme was measured by optical absorption. We discuss the possibility of extending this lower limit to zeptomoles of enzyme.

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BenzothiazolesHorseradish PeroxidaseHydrogen PeroxideIndicators and ReagentsMicrofluidic Analytical TechniquesOptics and PhotonicsOxidation-ReductionPolystyrenesProteinsSulfonic AcidsSurface TensionJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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Kenneth T Kotz, Yu Gu, Gregory W Faris. 2005-04-27. Optically addressed droplet-based protein assay.. https://doi.org/10.1021/ja050249m

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