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Medium optimization by response surface methodology for poly-gamma-glutamic acid production using dairy manure as the basis of a solid substrate.

Chen Xiong·Chen Shouwen·Sun Ming·Yu Ziniu
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Abstract

Dairy manure, supplemented with agro-industrial materials, was used as the solid substrate for high yield of poly-gamma-glutamic acid (gamma-PGA) by Bacillus subtilis CCTCC202048. The solid-state fermentation medium was optimized by response surface methodology. In the first optimization step, a Plackett-Burman design was used to evaluate the influence of related factors. Wheat bran, soybean cake and glutamic acid were found to be more compatible supplement with dairy manure and positively influenced on gamma-PGA production. In the second step, the concentrations of the three supplemental nutrients above were further optimized using a Box-Behnken design. The average gamma-PGA yield (4.70%) in triplicate under optimal conditions was obtained on the laboratory scale, whereas it was 3.58% at compost experiment. These would lay a foundation for lessening the pollution of dairy manure, increasing fertilizer efficiency and exploring a late-model organic fertilizer that retains water and nutrients.

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AnimalsBacillus subtilisBacteriological TechniquesCattleColony Count, MicrobialCulture MediaDairyingFermentationIndustrial MicrobiologyManurePolyglutamic AcidSubstrate SpecificityEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Chen Xiong, Chen Shouwen, Sun Ming, Yu Ziniu. 2005-04-22. Medium optimization by response surface methodology for poly-gamma-glutamic acid production using dairy manure as the basis of a solid substrate.. https://doi.org/10.1007/s00253-005-1989-z

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