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Oligonucleotide microarray for HLA-DRB1 genotyping: preparation and clinical evaluation.

F Zhang·S W Hu·J Huang·H Wang·W Zhang·Y Y Geng·S Q Wang
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Abstract

In this study, HLA-DRB1 gene was genotyped by using the microarray technique. Oligonucleotide probes were designed based on partial sequences of various genotypes of HLA-DRB1, and were fixed on a silylated slide to form a microarray. The second exon of HLA-DRB1 gene in the extracted genomic DNA samples was amplified and labelled by means of polymerase chain reaction (PCR); then it was hybridized to the microarray. The microarray was scanned, and the result was analysed in order to determine the genotypes of HLA-DRB1 of the tested sample. A total of 1574 of 1592 clinical samples had accordant results of genotypes in either microarray assay or PCR-SSP assay; 8 of 10 samples that had inconsistent results of genotypes were proved to be microarray-assay reliable by confirmation of DNA sequencing. It is concluded that microarray is an alternative reliable method for HLA-DRB1 genotyping.

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ChinaExonsGene FrequencyGenes, MHC Class IIGenotypeHLA-DR AntigensHLA-DRB1 ChainsHistocompatibility TestingHumansOligonucleotide Array Sequence AnalysisOligonucleotide ProbesPolymerase Chain ReactionReproducibility of ResultsSensitivity and SpecificityTemplates, GeneticComparative StudyEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov't

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F Zhang, S W Hu, J Huang, H Wang, W Zhang, Y Y Geng, S Q Wang. 2005. Oligonucleotide microarray for HLA-DRB1 genotyping: preparation and clinical evaluation.. https://doi.org/10.1111/j.1399-0039.2005.00395.x

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