Interface for direct and continuous sample-matrix deposition onto a MALDI probe for polymer analysis by thermal field flow fractionation and off-line MALDI-MS.

Franco Basile; Galina E Kassalainen; S Kim Ratanathanawongs Williams

doi:10.1021/ac048391d

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Interface for direct and continuous sample-matrix deposition onto a MALDI probe for polymer analysis by thermal field flow fractionation and off-line MALDI-MS.

Franco Basile·Galina E Kassalainen·S Kim Ratanathanawongs Williams

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Abstract

A simple interface based on an oscillating capillary nebulizer (OCN) is described for direct deposition of eluate from a thermal field-flow fractionation (ThFFF) system onto a matrix-assisted laser desorption/ionization (MALDI) probe. In this study, the polymer-containing eluent from the ThFFF system was mixed on-line with MALDI matrix solution and deposited directly onto a moving MALDI probe. The result was a continuous sample track representative of the fractionation process. Subsequent off-line MALDI-mass spectrometry analysis was performed in automated and manual modes. Polystyrene samples of broad polydispersity were used to characterize the overall system performance. The OCN interface is easy to build and operate without the use of heaters or high voltages and is compatible with any MALDI probe format.

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Fractionation, Field Flow Polystyrenes Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

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Franco Basile, Galina E Kassalainen, S Kim Ratanathanawongs Williams. 2005-05-01. Interface for direct and continuous sample-matrix deposition onto a MALDI probe for polymer analysis by thermal field flow fractionation and off-line MALDI-MS.. https://doi.org/10.1021/ac048391d

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