Physical coupling between ryanodine receptor-calcium release channels.

Chang-Cheng Yin; Lynda M Blayney; F Anthony Lai

doi:10.1016/j.jmb.2005.04.002

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Physical coupling between ryanodine receptor-calcium release channels.

Chang-Cheng Yin·Lynda M Blayney·F Anthony Lai

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Abstract

Ryanodine receptor-calcium release channels play a pivotal role in the calcium signaling that mediates muscle excitation-contraction coupling. Their membrane organization into regular patterns, functional gating studies and theoretical analysis of receptor clustering have led to models that invoke allosteric interaction between individual channel oligomers as a critical mechanism for control of calcium release. Here we show that in reconstituted "checkerboard-like" lattices that mimic in situ membrane channel arrays, each oligomer is interlocked physically with four adjacent oligomers via a specific domain-domain interaction. Direct physical coupling between ryanodine receptors provides structural evidence for an inter-oligomer allosteric mechanism in channel regulation. Therefore, in addition to established cytosolic and luminal regulation of function, these observations indicate that channel-channel communication through physical coupling provides a novel mode of regulation of intracellular calcium release channels.

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Animals Calcium Signaling Models, Molecular Protein Structure, Tertiary Rabbits Ryanodine Receptor Calcium Release Channel Sarcoplasmic Reticulum Journal Article Research Support, Non-U.S. Gov't

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Chang-Cheng Yin, Lynda M Blayney, F Anthony Lai. 2005-04-25. Physical coupling between ryanodine receptor-calcium release channels.. https://doi.org/10.1016/j.jmb.2005.04.002

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