A new mathematical function for describing electrophoretic peaks.

Abstract

A new model is proposed for characterizing skewed electrophoretic peaks, which is a combination of leading and trailing edge functions, empirically modified to get a rapid recovery of the baseline. The peak model is a sum of square roots and is called thereby "combined square roots (CSR) model". The flexibility of the model was checked on theoretical and experimental peaks with asymmetries in the range of 0-10 (expressed as the ratio of the distance between the center and the trailing edge, and the center and the leading edge of the chromatographic peak, measured at 10% of peak height). Excellent fits were found in all cases. The new model was compared with other three models that have shown good performance in modelling chromatographic peaks: the empirically transformed Gaussian, the parabolic Lorentzian-modified Gaussian, and the Haarhoff-van der Linde function. The latter model was proposed recently to describe electrophoretic peaks. The CSR model offered the highest flexibility to describe electrophoretic peak profiles, even those extremely asymmetrical with long tails. The new function has the advantage of using measurable parameters that allow the direct estimation of peak areas, which is useful for quantitative purposes.