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PubMed · 15883035

Polymorphisms in human pre-miRNAs.

Naoharu Iwai·Hiroaki Naraba
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Abstract

MicroRNAs constitute a growing class of non-coding RNAs that are thought to regulate gene expression via translational repression. MicroRNAs are initially transcribed as several hundred-nucleotide pri-miRNAs and are then processed to approximately 60-nucleotide hairpin pre-miRNAs. We hypothesized that polymorphisms in both pre-miRNA and mature microRNA modify various biological processes by influencing the processing and/or target selection of microRNAs. In the present study, we sequenced 173 human pre-miRNA genome regions in 96 subjects and found 10 polymorphisms in the 10 pre-miRNA hairpin regions. Although most of these polymorphisms seem to have no effect on microRNA processing, we identified a C to A polymorphism in the mature miR-30c-2 sequence. This polymorphism may alter target selection and thus exert profound biological effects. To the best of our knowledge, this is the first report of polymorphisms in pre-miRNAs.

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Base SequenceBlotting, NorthernHumansMicroRNAsMolecular Sequence DataNucleic Acid ConformationPolymorphism, GeneticRNA PrecursorsSequence Homology, Nucleic AcidJournal ArticleResearch Support, Non-U.S. Gov't

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Naoharu Iwai, Hiroaki Naraba. 2005-06-17. Polymorphisms in human pre-miRNAs.. https://doi.org/10.1016/j.bbrc.2005.04.051

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