A highly sensitive high-performance liquid chromatography-mass spectrometry method for quantification of fludarabine triphosphate in leukemic cells.

Thomas F Kalhorn; Aaron G Ren; John T Slattery; Jeannine S McCune; Joanne Wang

doi:10.1016/j.jchromb.2005.03.034

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A highly sensitive high-performance liquid chromatography-mass spectrometry method for quantification of fludarabine triphosphate in leukemic cells.

Thomas F Kalhorn·Aaron G Ren·John T Slattery·Jeannine S McCune·Joanne Wang

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Abstract

A high-performance liquid chromatography (HPLC)-mass spectrometry (MS) method has been developed for the analysis of 9-beta-D-arabinofuranosyl-2-fluoroadenine 5'-triphosphate (F-ara-ATP) from biological samples. Quantification is carried out by selected ion monitoring of the parent ion. Baseline separation of the monophosphate (F-ara-AMP) and diphosphate (F-ara-ADP) is achieved using the volatile ion-pairing reagent dimethylhexylamine. This method is selective and sensitive with an on-column detection limit of approximately 50 fmol. It also permits simultaneous monitoring of endogenous adenosine phosphates. The utility of the assay has been demonstrated by the analysis of F-ara-ATP in human leukemic cells after incubation with 9-beta-D-arabinosyl-2-fluoroadenine (F-ara-A) at clinically relevant concentrations.

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Antineoplastic Agents Chromatography, High Pressure Liquid Humans Jurkat Cells Leukemia Mass Spectrometry Reference Standards Reproducibility of Results Sensitivity and Specificity Vidarabine Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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Thomas F Kalhorn, Aaron G Ren, John T Slattery, Jeannine S McCune, Joanne Wang. 2005-04-21. A highly sensitive high-performance liquid chromatography-mass spectrometry method for quantification of fludarabine triphosphate in leukemic cells.. https://doi.org/10.1016/j.jchromb.2005.03.034

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