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PubMed · 15900449

Individual cell migration analysis using fiber-optic bundles.

Christopher DiCesare·Israel Biran·David R Walt
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Abstract

In this paper we describe a novel optical fiber-based technology for analyzing cell migration. Cells were labeled with a membrane-bound fluorescent dye and distributed onto a polished optical fiber bundle. When a cell passes over one of the individual fibers in the bundle, the membrane-bound dye causes a large intensity increase, which stays for a given "residence time" until the cell departs from the fiber. Residence time increases significantly upon exposure to an antimigratory drug, indicating a decrease in cell migration. This approach provides a simple migration assay and does not require sophisticated tracking software. By using optical fiber bundles containing smaller individual fibers with higher spatial resolution, this approach was employed to develop a migration assay based on subcellular imaging. The subcellular imaging platform allows for rapid analysis of migratory potential, reducing experimental time from several hours in a standard assay to 5 min using this technology.

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Cell MovementFiber Optic TechnologyOptical FibersSpectrometry, FluorescenceJournal ArticleResearch Support, N.I.H., Extramural

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BibTeXRIS

Christopher DiCesare, Israel Biran, David R Walt. 2005-04-01. Individual cell migration analysis using fiber-optic bundles.. https://doi.org/10.1007/s00216-005-3142-3

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