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Macrophage migration inhibitory factor expression correlates with inflammatory changes in human chronic hepatitis B infection.

Hai-Ying Zhang·Amin A Nanji·John M Luk·Xiao-Ru Huang·Chung-Mau Lo·Yong Xiong Chen·Siu-Tsan Yuen·Hui Y Lan·George K K Lau
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Abstract

BACKGROUND: Macrophage migration inhibitory factor (MIF) has emerged to be a pivotal cytokine in immune-mediated diseases. PATIENTS AND METHODS: To investigate the role of MIF in chronic hepatitis B infection, we studied two groups of hepatitis B surface antigen positive patients: group 1 (immune tolerant, n = 16) and group 2 (immune clearance, n = 16). Serum level of MIF was measured by enzyme-linked immunosorbent assay and intrahepatic expression of MIF, macrophage and T-cell localisation were detected by double immunohistochemistry. RESULTS: An increased serum MIF correlated significantly with increased serum alanine aminotransferase activity (r = 0.73, P < 0.001) and the severity of necroinflammatory injury (r = 0.642, P < 0.001). In group 2, there was marked MIF mRNA expression in all KP-1+ macrophages and CD45RO+ activated T cells and, to a lesser extent, in hepatocytes within inflammatory areas. In contrast to its mRNA expression, the cytoplasmic MIF protein level in hepatocytes, infiltrating macrophages and T cells within the inflammatory area was reduced, which probably contributed to the increased serum MIF level. CONCLUSIONS: Our data suggested that MIF played a role in sustaining cell-mediated hepatic injury during the immune-clearance phase of chronic hepatitis B infection.

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AdultFemaleGene ExpressionHepatitis B e AntigensHepatitis B, ChronicHumansImmune ToleranceLiverMacrophage Migration-Inhibitory FactorsMacrophagesMaleMiddle AgedNecrosisRNA, MessengerT-LymphocytesJournal ArticleResearch Support, Non-U.S. Gov't

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Hai-Ying Zhang, Amin A Nanji, John M Luk, Xiao-Ru Huang, Chung-Mau Lo, Yong Xiong Chen, Siu-Tsan Yuen, Hui Y Lan, George K K Lau. 2005. Macrophage migration inhibitory factor expression correlates with inflammatory changes in human chronic hepatitis B infection.. https://doi.org/10.1111/j.1478-3231.2005.01047.x

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