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Gene expression profiling to characterize anticancer drug sensitivity.

James K Breaux·Gerrit Los
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Abstract

This chapter presents a protocol for using cDNA microarrays to acquire gene expression profiles that characterize anticancer drug sensitivity. The protocol includes steps for drug exposure, RNA isolation, preparation of fluorescently labeled samples, microarray hybridization, data processing, and data analysis. In addition to the detailed protocol, important experimental design issues are discussed, and some preliminary experiments are recommended.

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Antineoplastic AgentsCell Line, TumorDNA, ComplementaryDrug Screening Assays, AntitumorFluorescent DyesGene Expression ProfilingGene Expression Regulation, NeoplasticHumansNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisRNA, NeoplasmStatistics as TopicTime FactorsToxicogeneticsJournal Article

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James K Breaux, Gerrit Los. 2005. Gene expression profiling to characterize anticancer drug sensitivity.. https://doi.org/10.1385/1-59259-889-7%3A197

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