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Supercritical fluid chromatography tandem-column method development in pharmaceutical sciences for a mixture of four stereoisomers.

Wesley W Barnhart·Kyung H Gahm·Sam Thomas·Steve Notari·David Semin·Janet Cheetham
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Abstract

A tandem-column method using Chiralpak AD-H and Chiralcel OD-H columns was achieved for baseline separation of a mixture of chiral pharmaceutical compounds (i.e., four stereoisomers) via supercritical fluid chromatography (SFC) with a mobile phase consisting of 90% liquid carbon dioxide and 10% ethanol:isopropanol (50:50 v/v). On the contrary, this mixture (mixture A) could not be baseline separated by SFC conditions explored with individual Chiralpak AD-H and Chiralcel OD-H columns. The effects of various mobile phases on elution order, capacity factor, selectivity, and resolution were determined with mixture A on the individual aforementioned columns to develop the tandem-column method.

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Chromatography, LiquidPharmaceutical PreparationsStereoisomerismTechnology, PharmaceuticalJournal Article

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Wesley W Barnhart, Kyung H Gahm, Sam Thomas, Steve Notari, David Semin, Janet Cheetham. 2005. Supercritical fluid chromatography tandem-column method development in pharmaceutical sciences for a mixture of four stereoisomers.. https://doi.org/10.1002/jssc.200500005

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