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Co-expressed recombinant human Translin-Trax complex binds DNA.

Gagan D Gupta·Ravindra D Makde·Radhika P Kamdar·Jacinta S D'Souza·Meeta G Kulkarni·Vinay Kumar·Basuthkar J Rao
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Abstract

Trax, expressed alone aggregates into insoluble complexes, whereas upon co-expression with Translin becomes readily soluble and forms a stable heteromeric complex ( approximately 430 kDa) containing both proteins at nearly equimolar ratio. Based on the subunit molecular weights, estimated by MALDI-TOF-MS, the purified complex appears to comprise of either an octameric Translin plus a hexameric Trax (calculated MW 420 kDa) or a heptamer each of Trax and Translin (calculated MW 425 kDa) or a hexameric Translin plus an octameric Trax (calculated MW 431 kDa). The complex binds single-stranded/double-stranded DNA. ssDNA gel-shifted complex shows both proteins at nearly equimolar ratio, suggesting that Translin "chaperones" Trax and forms heteromeric complex that is DNA binding competent.

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DNADNA-Binding ProteinsEscherichia coliGene ExpressionHumansMultiprotein ComplexesProtein BindingRecombinant ProteinsJournal Article

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Gagan D Gupta, Ravindra D Makde, Radhika P Kamdar, Jacinta S D'Souza, Meeta G Kulkarni, Vinay Kumar, Basuthkar J Rao. 2005-06-06. Co-expressed recombinant human Translin-Trax complex binds DNA.. https://doi.org/10.1016/j.febslet.2005.05.007

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