MicroRNA expression in zebrafish embryonic development.

Erno Wienholds; Wigard P Kloosterman; Eric Miska; Ezequiel Alvarez-Saavedra; Eugene Berezikov; Ewart de Bruijn; H Robert Horvitz; Sakari Kauppinen; Ronald H A Plasterk

doi:10.1126/science.1114519

PubMed · 15919954

MicroRNA expression in zebrafish embryonic development.

Erno Wienholds·Wigard P Kloosterman·Eric Miska·Ezequiel Alvarez-Saavedra·Eugene Berezikov·Ewart de Bruijn·H Robert Horvitz·Sakari Kauppinen·Ronald H A Plasterk

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Abstract

MicroRNAs (miRNAs) are small noncoding RNAs, about 21 nucleotides in length, that can regulate gene expression by base-pairing to partially complementary mRNAs. Regulation by miRNAs can play essential roles in embryonic development. We determined the temporal and spatial expression patterns of 115 conserved vertebrate miRNAs in zebrafish embryos by microarrays and by in situ hybridizations, using locked-nucleic acid-modified oligonucleotide probes. Most miRNAs were expressed in a highly tissue-specific manner during segmentation and later stages, but not early in development, which suggests that their role is not in tissue fate establishment but in differentiation or maintenance of tissue identity.

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Animals Blotting, Northern Embryo, Nonmammalian Embryonic Development Gene Expression In Situ Hybridization MicroRNAs Multigene Family Oligonucleotide Array Sequence Analysis Oligonucleotide Probes Organ Specificity Time Factors Zebrafish Journal Article Research Support, Non-U.S. Gov't

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Erno Wienholds, Wigard P Kloosterman, Eric Miska, Ezequiel Alvarez-Saavedra, Eugene Berezikov, Ewart de Bruijn, H Robert Horvitz, Sakari Kauppinen, Ronald H A Plasterk. 2005-05-26. MicroRNA expression in zebrafish embryonic development.. https://doi.org/10.1126/science.1114519

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