Laser-induced fluorescence microscopic system using an optical parametric oscillator for tunable detection in microchip analysis.

Momoko Kumemura; Tamao Odake; Takashi Korenaga

doi:10.1007/s00216-005-3211-7

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Laser-induced fluorescence microscopic system using an optical parametric oscillator for tunable detection in microchip analysis.

Momoko Kumemura·Tamao Odake·Takashi Korenaga

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Abstract

A laser-induced fluorescence microscopic system based on optical parametric oscillation has been constructed as a tunable detector for microchip analysis. The detection limit of sulforhodamine B (Ex. 520 nm, Em. 570 nm) was 0.2 mumol, which was approximately eight orders of magnitude better than with a conventional fluorophotometer. The system was applied to the determination of fluorescence-labeled DNA (Ex. 494 nm, Em. 519 nm) in a microchannel and the detection limit reached a single molecule. These results showed the feasibility of this system as a highly sensitive and tunable fluorescence detector for microchip analysis.

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Bacteriophage lambda DNA, Viral Fluorescence Lab-On-A-Chip Devices Lasers Microchip Analytical Procedures Microscopy, Fluorescence Optics and Photonics Rhodamines Sensitivity and Specificity Journal Article Research Support, Non-U.S. Gov't

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Momoko Kumemura, Tamao Odake, Takashi Korenaga. 2005-06-01. Laser-induced fluorescence microscopic system using an optical parametric oscillator for tunable detection in microchip analysis.. https://doi.org/10.1007/s00216-005-3211-7

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