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PubMed · 15941587

A new plasmid vector for regulated gene expression in Bacillus subtilis.

Gemma Serrano-Heras·Margarita Salas·Alicia Bravo
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Abstract

We have developed a novel regulated expression vector for Bacillus subtilis based on the Staphylococcus aureus plasmid pUB110. This vector, named pPR54, carries the P(R) promoter and the cI857 gene (encoding a temperature-sensitive transcriptional repressor) from the Escherichia coli phage lambda. Using the gfp gene from the jellyfish Aequorea victoria as a reporter, we show that pPR54 is a useful vector for controllable production of heterologous proteins in B. subtilis.

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Bacillus subtilisBacterial ProteinsChromosome MappingFluorescenceGene Expression Regulation, BacterialGenes, BacterialGenetic VectorsGreen Fluorescent ProteinsPlasmidsPromoter Regions, GeneticRepressor ProteinsTemperatureJournal ArticleResearch Support, Non-U.S. Gov't

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Gemma Serrano-Heras, Margarita Salas, Alicia Bravo. 2005-06-06. A new plasmid vector for regulated gene expression in Bacillus subtilis.. https://doi.org/10.1016/j.plasmid.2005.04.002

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