Spectral-domain phase microscopy.

Michael A Choma; Audrey K Ellerbee; Changhuei Yang; Tony L Creazzo; Joseph A Izatt

doi:10.1364/ol.30.001162

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Spectral-domain phase microscopy.

Michael A Choma·Audrey K Ellerbee·Changhuei Yang·Tony L Creazzo·Joseph A Izatt

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Abstract

Broadband interferometry is an attractive technique for the detection of cellular motions because it provides depth-resolved phase information via coherence gating. We present a phase-sensitive technique called spectral-domain phase microscopy (SDPM). SDPM is a functional extension of spectral-domain optical coherence tomography that allows for the detection of nanometer-scale motions in living cells. The sensitivity of the technique is demonstrated, and its calibration is verified. A shot-noise limit to the displacement sensitivity of this technique is derived. Measurement of cellular dynamics was performed on spontaneously beating cardiomyocytes isolated from chick embryos.

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Animals Cell Movement Cells, Cultured Chick Embryo Equipment Design Equipment Failure Analysis Microscopy, Interference Microscopy, Phase-Contrast Myocardial Contraction Myocytes, Cardiac Tomography, Optical Coherence Evaluation Study Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

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Michael A Choma, Audrey K Ellerbee, Changhuei Yang, Tony L Creazzo, Joseph A Izatt. 2005-05-15. Spectral-domain phase microscopy.. https://doi.org/10.1364/ol.30.001162

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