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Genes transactivated by hepatitis C virus core protein, a microarray assay.

Min Liu·Shu-Lin Zhang·Jun Cheng·Yan Liu·Lin Wang·Qing Shao·Jian Zhang·Shu-Mei Lin
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Abstract

AIM: To explore the new target genes transactivated by hepatitis C virus (HCV) core protein and to elucidate the pathogenesis of HCV infection. METHODS: Reverse transcribed cDNA was subjected to microarray assay. The coding gene transactivated by HCV core protein was cloned and analyzed with bioinformatics methods. RESULTS: The expressive vector of pcDNA3.1(-)-core was constructed and confirmed by restriction enzyme digestion and DNA sequencing and approved correct. mRNA was purified from HepG2 and HepG2 cells transfected with pcDNA3.1(-)-core, respectively. The cDNA derived was subjected to microarray assay. A new gene named HCTP4 was cloned with molecular biological method in combination with bioinformatics method. CONCLUSION: HCV core is a potential transactivator. Microarray is an efficient and convenient method for analysis of differentially expressed genes.

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Carcinoma, HepatocellularCell Line, TumorGene Expression Regulation, ViralHepacivirusHepatitis CHumansLiver NeoplasmsOligonucleotide Array Sequence AnalysisJournal ArticleResearch Support, Non-U.S. Gov't

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Min Liu, Shu-Lin Zhang, Jun Cheng, Yan Liu, Lin Wang, Qing Shao, Jian Zhang, Shu-Mei Lin. 2005-06-14. Genes transactivated by hepatitis C virus core protein, a microarray assay.. https://doi.org/10.3748/wjg.v11.i22.3351

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