[Application with single-stranded probes in randomized terminal linker-dependent PCR].

Chao-long Rao; Qing Wu; Zheng-chang Heng

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PubMed · 15952655

[Application with single-stranded probes in randomized terminal linker-dependent PCR].

Chao-long Rao·Qing Wu·Zheng-chang Heng

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Abstract

OBJECTIVE: Preparing single-stranded (ss) probes in place of double-stranded (ds) probes to improve the hybridization efficiency and specificity of randomized terminal linker-dependent PCR (RDPCR). METHODS: Using asymmetric PCR and single-primer PCR to prepare ss probes of extron 7 of p53 gene in rat, then comparing the results hybridized with ss probes and ds probes. RESULTS: Preparation of ss probes by asymmetric PCR and single-primer PCR gets success. Hybridization results showed that the ss probes could get better signals and less noise than ds probes. CONCLUSION: In comparison with ds probes, the application of ss probes can increase the hybridization sensitivity and specificity of RDPCR.

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Animals DNA Primers DNA Probes Exons Genes, p53 Hybridization, Genetic Male Polymerase Chain Reaction Rats Rats, Sprague-Dawley Sensitivity and Specificity English Abstract Journal Article Research Support, Non-U.S. Gov't

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Chao-long Rao, Qing Wu, Zheng-chang Heng. 2005. [Application with single-stranded probes in randomized terminal linker-dependent PCR].. https://pubmed.ncbi.nlm.nih.gov/15952655/

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