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Direct interaction between SNAP-23 and L-type Ca2+ channel.

Won Jin Cho·Aleksandar Jeremic·Bhanu P Jena
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Abstract

During secretion, membrane-bound secretory vesicles dock and fuse at the base of porosomes in the cell plasma membrane. Among other proteins, the porosome is composed of SNAREs and Ca2+-channels. Ca2+-channels and SNAREs have been implicated in cell secretion. Several immunoprecipitation and binding studies suggest the physical interaction of the t-SNARE proteins, Syntaxin-1 and SNAP-25 with various Ca2+-channels. In this study, using yeast two-hybrid and immunoanalysis, we demonstrate for the first time, direct interaction of SNAP-23 and a L-type Ca2+-channel at the plasma membrane in pancreas.

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Amino Acid SequenceAnimalsBase SequenceCalcium Channels, L-TypeCarrier ProteinsCell MembraneImmunoprecipitationMaleModels, MolecularPancreasProtein BindingQb-SNARE ProteinsQc-SNARE ProteinsRatsRats, Sprague-DawleySequence Homology, Amino AcidSequence Homology, Nucleic AcidTwo-Hybrid System TechniquesYeastsJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, U.S. Gov't, P.H.S.

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Won Jin Cho, Aleksandar Jeremic, Bhanu P Jena. Direct interaction between SNAP-23 and L-type Ca2+ channel.. https://doi.org/10.1111/j.1582-4934.2005.tb00363.x

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