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PubMed · 15966810

Characterization and quantitation of membrane proteomes using multidimensional MS-based proteomic technologies.

Josip Blonder·Thomas P Conrads·Timothy D Veenstra
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Abstract

A major goal of proteomics is to develop methods that enable the systematic characterization of every protein within the cell or particular subcellular proteome using a single analytical platform. Although the equivalent has already been achieved in genomics, reaching this goal in proteomics represents a much greater challenge due to the wide dynamic range of protein expression, numerous post-translational modifications and remarkable physicochemical heterogeneity of proteins. A major analytical challenge has involved developing more effective means for proteome-scale investigations of membrane proteins, whose solubility differs drastically from that of cytoplasmic proteins. Fortunately, rapid progress has increased the ability to characterize this critically important class of proteins on a scale analogous to that of aqueous soluble proteins.

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Cell AdhesionMembrane ProteinsModels, MolecularProtein ConformationProteinsProteomicsSolubilitySpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, U.S. Gov't, P.H.S.Review

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Josip Blonder, Thomas P Conrads, Timothy D Veenstra. 2004. Characterization and quantitation of membrane proteomes using multidimensional MS-based proteomic technologies.. https://doi.org/10.1586/14789450.1.2.153

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