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PubMed · 15968513

DNA methylation increases throughout Arabidopsis development.

L Ruiz-García·M T Cervera·J M Martínez-Zapater
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Abstract

We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs.

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ArabidopsisDNA MethylationDNA, PlantGene Expression Regulation, DevelopmentalGene Expression Regulation, PlantJournal ArticleResearch Support, Non-U.S. Gov't

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L Ruiz-García, M T Cervera, J M Martínez-Zapater. 2005-06-21. DNA methylation increases throughout Arabidopsis development.. https://doi.org/10.1007/s00425-005-1524-6

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