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PubMed · 15977067

Fast screening target sites for RNA interference using a cell-free system.

Huang Mei·Jia Fang-Jun·Li Chong-Yong·Li Yi-Ping·Guo Li-He
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Abstract

A fast and simple procedure to screen target sites for RNA interference by using RNA in a cell-free system of Hela cells, and then evaluating the efficiency by Northern blotting, is described. This procedure produces results with an identical reliability compared to those described previously but which are more time-consuming than this present method.

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BiotechnologyBlotting, NorthernCell-Free SystemGenetic TechniquesGreen Fluorescent ProteinsHeLa CellsHumansNucleic Acid HybridizationPlasmidsRNA InterferenceRNA, Small InterferingTemperatureTime FactorsTransfectionJournal Article

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Huang Mei, Jia Fang-Jun, Li Chong-Yong, Li Yi-Ping, Guo Li-He. 2005. Fast screening target sites for RNA interference using a cell-free system.. https://doi.org/10.1007/s10529-005-3627-3

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