Issues in the analysis of oligonucleotide tiling microarrays for transcript mapping.

Thomas E Royce; Joel S Rozowsky; Paul Bertone; Manoj Samanta; Viktor Stolc; Sherman Weissman; Michael Snyder; Mark Gerstein

doi:10.1016/j.tig.2005.06.007

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Issues in the analysis of oligonucleotide tiling microarrays for transcript mapping.

Thomas E Royce·Joel S Rozowsky·Paul Bertone·Manoj Samanta·Viktor Stolc·Sherman Weissman·Michael Snyder·Mark Gerstein

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Abstract

Traditional microarrays use probes complementary to known genes to quantitate the differential gene expression between two or more conditions. Genomic tiling microarray experiments differ in that probes that span a genomic region at regular intervals are used to detect the presence or absence of transcription. This difference means the same sets of biases and the methods for addressing them are unlikely to be relevant to both types of experiment. We introduce the informatics challenges arising in the analysis of tiling microarray experiments as open problems to the scientific community and present initial approaches for the analysis of this nascent technology.

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Algorithms Base Sequence Computational Biology DNA Humans Molecular Probes Oligonucleotide Array Sequence Analysis Transcription, Genetic NASA Center ARC NASA Discipline Life Sciences Technologies Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.Review

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Thomas E Royce, Joel S Rozowsky, Paul Bertone, Manoj Samanta, Viktor Stolc, Sherman Weissman, Michael Snyder, Mark Gerstein. 2005. Issues in the analysis of oligonucleotide tiling microarrays for transcript mapping.. https://doi.org/10.1016/j.tig.2005.06.007

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