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OrfPredictor: predicting protein-coding regions in EST-derived sequences.

Xiang Jia Min·Gregory Butler·Reginald Storms·Adrian Tsang
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Abstract

OrfPredictor is a web server designed for identifying protein-coding regions in expressed sequence tag (EST)-derived sequences. For query sequences with a hit in BLASTX, the program predicts the coding regions based on the translation reading frames identified in BLASTX alignments, otherwise, it predicts the most probable coding region based on the intrinsic signals of the query sequences. The output is the predicted peptide sequences in the FASTA format, and a definition line that includes the query ID, the translation reading frame and the nucleotide positions where the coding region begins and ends. OrfPredictor facilitates the annotation of EST-derived sequences, particularly, for large-scale EST projects. OrfPredictor is available at https://fungalgenome.concordia.ca/tools/OrfPredictor.html.

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AlgorithmsAmino Acid SequenceCodonDatabases, GeneticExpressed Sequence TagsInternetOpen Reading FramesSequence Analysis, DNASoftwareUser-Computer InterfaceEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Xiang Jia Min, Gregory Butler, Reginald Storms, Adrian Tsang. 2005-07-01. OrfPredictor: predicting protein-coding regions in EST-derived sequences.. https://doi.org/10.1093/nar%2Fgki394

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