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PBAF chromatin-remodeling complex requires a novel specificity subunit, BAF200, to regulate expression of selective interferon-responsive genes.

Zhijiang Yan·Kairong Cui·Darryl M Murray·Chen Ling·Yutong Xue·Amy Gerstein·Ramon Parsons·Keji Zhao·Weidong Wang
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Abstract

PBAF and BAF are two chromatin-remodeling complexes of the SWI/SNF family essential for mammalian transcription and development. Although these complexes share eight identical subunits, only PBAF can facilitate transcriptional activation by nuclear receptors in vitro. Here we show that these complexes have selectivity in mediating transcription of different interferon-responsive genes. The selectivity by PBAF requires a novel subunit, BAF200, but not the previously described PBAF-specificity subunit, BAF180 (Polybromo). Our study provides in vivo evidence that PBAF and BAF regulate expression of distinct genes, and suggests that BAF200 plays a key role in PBAF function.

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Chromatin Assembly and DisassemblyChromosomal Proteins, Non-HistoneGene Expression RegulationHeLa CellsHumansIn Vitro TechniquesInterferon Type IMultiprotein ComplexesProtein SubunitsRNA, Small InterferingRecombinant ProteinsTranscription FactorsJournal ArticleResearch Support, Non-U.S. Gov't

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Zhijiang Yan, Kairong Cui, Darryl M Murray, Chen Ling, Yutong Xue, Amy Gerstein, Ramon Parsons, Keji Zhao, Weidong Wang. 2005-06-28. PBAF chromatin-remodeling complex requires a novel specificity subunit, BAF200, to regulate expression of selective interferon-responsive genes.. https://doi.org/10.1101/gad.1323805

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