Bone marrow cell-seeded biodegradable polymeric scaffold enhances angiogenesis and improves function of the infarcted heart.

Shinya Fukuhara; Shinji Tomita; Takeshi Nakatani; Toshiya Fujisato; Yoshinori Ohtsu; Michiko Ishida; Chikao Yutani; Soichiro Kitamura

doi:10.1253/circj.69.850

PubMed · 15988112

Bone marrow cell-seeded biodegradable polymeric scaffold enhances angiogenesis and improves function of the infarcted heart.

Shinya Fukuhara·Shinji Tomita·Takeshi Nakatani·Toshiya Fujisato·Yoshinori Ohtsu·Michiko Ishida·Chikao Yutani·Soichiro Kitamura

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Abstract

BACKGROUND: The present study examined whether a bioengineered polyglycolic acid cloth (PGAC) impregnated with bone marrow cells (BMC) improved the function and angiogenesis of the infarcted heart. METHODS AND RESULTS: The coronary artery was ligated in Lewis rats and the infarcted area was covered with a PGAC in group 1 (n=8), with a PGAC containing basic-fibroblast growth factor (b-FGF) in group 2 (n=11) and a PGAC containing b-FGF and freshly isolated BMC in group 3 (n=10). In addition, BMC derived from transgenic mice expressing green fluorescent protein (GFP)-BMC were seeded into a PGAC, which was sutured over the infarcted area of C57BL/6 mice (n=5). In the rat study, developed and systolic pressures, dp/dt max and dp/dt min) were the highest in group 3, as were the capillary density in the PGAC and infarcted area. In the mouse study, there were few GFP-BMC in the PGAC, but none in the infarcted area. CONCLUSIONS: A PGAC with BMC improved cardiac function by inducing angiogenesis without migration of BMC. Freshly isolated BMC work as angiogenic inducers and a PGAC is useful as a "drug delivery system".

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Absorbable Implants Animals Bone Marrow Cells Mice Myocardial Infarction Myocardium Neovascularization, Physiologic Polyglycolic Acid Rats Rats, Inbred Lew Journal Article Research Support, Non-U.S. Gov't

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Shinya Fukuhara, Shinji Tomita, Takeshi Nakatani, Toshiya Fujisato, Yoshinori Ohtsu, Michiko Ishida, Chikao Yutani, Soichiro Kitamura. 2005. Bone marrow cell-seeded biodegradable polymeric scaffold enhances angiogenesis and improves function of the infarcted heart.. https://doi.org/10.1253/circj.69.850

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