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PubMed · 16000825

Multiple-mutation reaction: a method for simultaneous introduction of multiple mutations into the glpK gene of Mycoplasma pneumoniae.

Claudine Hames·Sven Halbedel·Oliver Schilling·Jörg Stülke
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Abstract

In Mycoplasma pneumoniae, the UGA opal codon specifies tryptophan rather than a translation stop site. This often makes it difficult to express Mycoplasma proteins in E. coli isolates. In this work, we developed a strategy for the one-step introduction of several mutations. This method, the multiple-mutation reaction, is used to simultaneously replace nine opal codons in the M. pneumoniae glpK gene.

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CodonEscherichia coliGene Expression Regulation, BacterialGenetic TechniquesGlycerol KinaseHumansMutagenesis, Site-DirectedMutationMycoplasma pneumoniaeEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Claudine Hames, Sven Halbedel, Oliver Schilling, Jörg Stülke. 2005. Multiple-mutation reaction: a method for simultaneous introduction of multiple mutations into the glpK gene of Mycoplasma pneumoniae.. https://doi.org/10.1128/aem.71.7.4097-4100.2005

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