Common-path phase-shift interferometry surface plasmon resonance imaging system.

Y D Su; S J Chen; T L Yeh

doi:10.1364/ol.30.001488

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Common-path phase-shift interferometry surface plasmon resonance imaging system.

Y D Su·S J Chen·T L Yeh

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Abstract

Surface plasmon resonance (SPR) and common-path phase-shift interferometry (PSI) techniques are integrated in a biosensing imaging system for measuring the two-dimensional spatial phase variation caused by biomolecular interactions on a sensing chip without the need for additional labeling. The common-path PSI technique has the advantage of long-term stability, even when it is subjected to external disturbances. Hence the system meets the requirements of the real-time kinetic studies involved in biomolecular interaction analysis. The proposed SPR-PSI imaging system demonstrates a detection limit of a 2 x 10(-7) refractive-index change, a long-term phase stability of 2.5 x 10(-4) pi rms for 4 h, and a spatial phase resolution of 10(-3) pi with a lateral resolution of 100 microm.

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Biosensing Techniques Equipment Design Equipment Failure Analysis Interferometry Oligonucleotide Array Sequence Analysis Refractometry Surface Plasmon Resonance Evaluation Study Journal Article

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Y D Su, S J Chen, T L Yeh. 2005-06-15. Common-path phase-shift interferometry surface plasmon resonance imaging system.. https://doi.org/10.1364/ol.30.001488

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