Lectin arrays for profiling cell surface carbohydrate expression.

Ting Zheng; Dora Peelen; Lloyd M Smith

doi:10.1021/ja0505550

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Lectin arrays for profiling cell surface carbohydrate expression.

Ting Zheng·Dora Peelen·Lloyd M Smith

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Abstract

We present a strategy for the analysis of cell surface carbohydrate expression patterns using lectin arrays fabricated on gold surfaces. Antibody and glycoprotein binding experiments showed that the lectins were effectively immobilized on the surface and retained their carbohydrate-binding specificities. The approach was demonstrated in the analysis of carbohydrate expression on two mammalian cell lines.

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Animals Antigens, Surface Carbohydrate Metabolism Carbohydrates Cell Line Cells Lectins Microarray Analysis Substrate Specificity Surface Properties Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

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Ting Zheng, Dora Peelen, Lloyd M Smith. 2005-07-20. Lectin arrays for profiling cell surface carbohydrate expression.. https://doi.org/10.1021/ja0505550

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