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PubMed · 16019190

NOX4 as an oxygen sensor to regulate TASK-1 activity.

Young-Mee Lee·Byung-Joo Kim·Yang-Sook Chun·Insuk So·Hyunsung Choi·Myung-Suk Kim·Jong-Wan Park
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Abstract

When oxygen sensing cells are excited by hypoxia, background K+ currents are inhibited. TASK-1, which is commonly expressed in oxygen sensing cells and makes a background K+ current, is inactivated by hypoxia. Thus TASK-1 is a candidate molecule responsible for hypoxic excitation. However, TASK-1 per se cannot sense oxygen and may require a regulatory protein that can. In the present study, we propose that the NADPH oxidase NOX4 functions as an oxygen-sensing partner and that it modulates the oxygen sensitivity of TASK-1. Confocal imaging revealed the co-localization of TASK-1 and NOX4 in the plasma membrane. In HEK293 cells expressing NOX4 endogenously, the activity of expressed TASK-1 was moderately inhibited by hypoxia, and this oxygen response was significantly augmented by NOX4. Moreover, the oxygen sensitivity of TASK-1 was abolished by NOX4 siRNA and NADPH oxidase inhibitors. These results suggest a novel function for NOX4 in the oxygen-dependent regulation of TASK-1 activity.

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Biosensing TechniquesCell LineCell MembraneEnzyme InhibitorsHumansHypoxiaHypoxia-Inducible Factor 1, alpha SubunitNADPH Oxidase 4NADPH OxidasesNerve Tissue ProteinsOnium CompoundsOxygenPotassiumPotassium Channels, Tandem Pore DomainRNA, Small InterferingSignal TransductionComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Young-Mee Lee, Byung-Joo Kim, Yang-Sook Chun, Insuk So, Hyunsung Choi, Myung-Suk Kim, Jong-Wan Park. 2005-07-14. NOX4 as an oxygen sensor to regulate TASK-1 activity.. https://doi.org/10.1016/j.cellsig.2005.05.025

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