Artificial polypeptide scaffold for protein immobilization.

Kechun Zhang; Michael R Diehl; David A Tirrell

doi:10.1021/ja051457h

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Artificial polypeptide scaffold for protein immobilization.

Kechun Zhang·Michael R Diehl·David A Tirrell

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Abstract

An artificial polypeptide scaffold composed of surface anchor and protein capture domains was designed and expressed in vivo. By using a mutant E. coli phenylalanyl-tRNA synthetase, the photoreactive amino acid para-azidophenylalanine was incorporated into the surface anchor domain. Octyltrichlorosilane-treated surfaces were functionalized with this polypeptide by spin coating and photocrosslinking. The resulting protein films were shown to immobilize recombinant proteins through association of coiled coil heterodimer.

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Amino Acid Sequence Escherichia coli Glutathione Transferase Green Fluorescent Proteins Leucine Zippers Molecular Sequence Data Peptides Phenylalanine-tRNA Ligase Protein Array Analysis Recombinant Proteins Silanes Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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Kechun Zhang, Michael R Diehl, David A Tirrell. 2005-07-27. Artificial polypeptide scaffold for protein immobilization.. https://doi.org/10.1021/ja051457h

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