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Isocratic high-performance liquid chromatographic method with ultraviolet detection for simultaneous determination of levels of voriconazole and itraconazole and its hydroxy metabolite in human serum.

GholamAli Khoschsorur·Franz Fruehwirth·Sieglinde Zelzer
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Abstract

A simple, specific method is presented for simultaneous determination of voriconazole and itraconazole and its metabolite, hydroxyitraconazole, in human serum using one-step liquid-liquid extraction and high-performance liquid chromatography. Linearity tests ranged from 0.1 to 8.0 microg/ml; the minimum detectable concentration was 0.03 microg/ml.

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Antifungal AgentsChromatography, High Pressure LiquidHumansItraconazolePyrimidinesReproducibility of ResultsSensitivity and SpecificityTriazolesUltraviolet RaysVoriconazoleEvaluation StudyJournal Article

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GholamAli Khoschsorur, Franz Fruehwirth, Sieglinde Zelzer. 2005. Isocratic high-performance liquid chromatographic method with ultraviolet detection for simultaneous determination of levels of voriconazole and itraconazole and its hydroxy metabolite in human serum.. https://doi.org/10.1128/aac.49.8.3569-3571.2005

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