An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plants.

Elmar Schmälzlin; Joost T van Dongen; Ingo Klimant; Bettina Marmodée; Martin Steup; Joachim Fisahn; Peter Geigenberger; Hans-Gerd Löhmannsröben

doi:10.1529/biophysj.105.063453

PubMed · 16049223

An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plants.

Elmar Schmälzlin·Joost T van Dongen·Ingo Klimant·Bettina Marmodée·Martin Steup·Joachim Fisahn·Peter Geigenberger·Hans-Gerd Löhmannsröben

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Abstract

A technique has been developed to measure absolute intracellular oxygen concentrations in green plants. Oxygen-sensitive phosphorescent microbeads were injected into the cells and an optical multifrequency phase-modulation technique was used to discriminate the sensor signal from the strong autofluorescence of the plant tissue. The method was established using photosynthesis-competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species.

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Chara Image Interpretation, Computer-Assisted Intracellular Fluid Luminescent Measurements Microscopy, Fluorescence Oxygen Oxygen Consumption Plants Evaluation Study Journal Article Research Support, Non-U.S. Gov't Validation Study

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Elmar Schmälzlin, Joost T van Dongen, Ingo Klimant, Bettina Marmodée, Martin Steup, Joachim Fisahn, Peter Geigenberger, Hans-Gerd Löhmannsröben. 2005. An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plants.. https://doi.org/10.1529/biophysj.105.063453

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