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Stage-dependent gene expression profiles during natural killer cell development.

Hyung-Sik Kang·Eun-Mi Kim·Sanggyu Lee·Suk-Ran Yoon·Toshihiko Kawamura·Young-Cheol Lee·Sangsoo Kim·Pyung-Keun Myung·San Ming Wang·Inpyo Choi
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Abstract

Natural killer (NK) cells develop from hematopoietic stem cells (HSCs) in the bone marrow. To understand the molecular regulation of NK cell development, serial analysis of gene expression (SAGE) was applied to HSCs, NK precursor (pNK) cells, and mature NK cells (mNK) cultured without or with OP9 stromal cells. From 170,464 total individual tags from four SAGE libraries, 35,385 unique genes were identified. A set of genes was expressed in a stage-specific manner: 15 genes in HSCs, 30 genes in pNK cells, and 27 genes in mNK cells. Among them, lipoprotein lipase induced NK cell maturation and cytotoxic activity. Identification of genome-wide profiles of gene expression in different stages of NK cell development affords us a fundamental basis for defining the molecular network during NK cell development.

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AnimalsCell DifferentiationCell LineGene Expression ProfilingGene LibraryHematopoietic Stem CellsKiller Cells, NaturalLipoprotein LipaseMiceComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Hyung-Sik Kang, Eun-Mi Kim, Sanggyu Lee, Suk-Ran Yoon, Toshihiko Kawamura, Young-Cheol Lee, Sangsoo Kim, Pyung-Keun Myung, San Ming Wang, Inpyo Choi. 2005-07-28. Stage-dependent gene expression profiles during natural killer cell development.. https://doi.org/10.1016/j.ygeno.2005.06.010

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