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Major role of soybean oil in preventing decrease in CYP4A1 mRNA content relative to PPARalpha mRNA down-regulation in infant rats.

Masuhiro Nishimura·Aiko Yamauchi·Mari Yamaguchi·Sanae Kishimoto·Nobuhiko Ueda·Shinsaku Naito
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Abstract

Changes in the levels of CYP4A1, PPARalpha, and RXRalpha mRNA expression in the liver following overdose of fat-free or fat-containing total parenteral nutrition (TPN) were studied in 3-week-old male Sprague-Dawley rats. The rats were divided into three groups: group 1, oral diet; group 2, fat-free TPN; and group 3, TPN with 20% of calories from soybean oil emulsion. Levels of CYP4A1, PPARalpha, and RXRalpha mRNA in the fat-free TPN group were significantly lower than those in the other groups. Levels of CYP4A1 and PPARalpha mRNA were strongly correlated (r=0.849), and levels of CYP4A1 and RXRalpha mRNA were weakly correlated (r=0.618). This is the first report of a strong correlation between the levels of CYP4A1 and PPARalpha mRNA following overdose of fat-free or fat-containing TPN in infant rats. Our results also indicate that it is important to include fat in TPN regimens in order to prevent CYP4A1 mRNA down-regulation, which may be related to changes in PPARalpha mRNA levels in the liver.

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AnimalsBase SequenceCytochrome P-450 Enzyme SystemCytochrome P450 Family 4DNA PrimersDown-RegulationMalePPAR alphaRNA, MessengerRatsRats, Sprague-DawleyRetinoid X Receptor alphaReverse Transcriptase Polymerase Chain ReactionSoybean OilJournal Article

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Masuhiro Nishimura, Aiko Yamauchi, Mari Yamaguchi, Sanae Kishimoto, Nobuhiko Ueda, Shinsaku Naito. 2005. Major role of soybean oil in preventing decrease in CYP4A1 mRNA content relative to PPARalpha mRNA down-regulation in infant rats.. https://doi.org/10.1248/bpb.28.1517

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