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Multilocus simple sequence repeat markers for differentiating strains and evaluating genetic diversity of Xylella fastidiosa.

Hong Lin·Edwin L Civerolo·Rong Hu·Samuel Barros·Marta Francis·M Andrew Walker
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Abstract

A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR primer design and were validated in PCR experiments. These multilocus SSR primers, distributed across the X. fastidiosa genome, clearly differentiated and clustered X. fastidiosa strains collected from grape, almond, citrus, and oleander. They are well suited for differentiating strains and studying X. fastidiosa epidemiology and population genetics.

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Base SequenceCitrusDNA PrimersGenetic MarkersGenetic VariationMinisatellite RepeatsNeriumPlant DiseasesPolymerase Chain ReactionPrunusSequence Analysis, DNAVitisXylellaEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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Hong Lin, Edwin L Civerolo, Rong Hu, Samuel Barros, Marta Francis, M Andrew Walker. 2005. Multilocus simple sequence repeat markers for differentiating strains and evaluating genetic diversity of Xylella fastidiosa.. https://doi.org/10.1128/aem.71.8.4888-4892.2005

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