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PubMed · 16091279

An improved colorimetric method for quantitation of heme using tetramethylbenzidine as substrate.

Nguyen Tien Huy·Dai Thi Xuan Trang·Dinh Thanh Uyen·Motohiro Sasai·Shigeharu Harada·Kaeko Kamei
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BenzidinesColorimetryHemeSensitivity and SpecificityJournal ArticleResearch Support, Non-U.S. Gov'tValidation Study

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Nguyen Tien Huy, Dai Thi Xuan Trang, Dinh Thanh Uyen, Motohiro Sasai, Shigeharu Harada, Kaeko Kamei. 2005-09-15. An improved colorimetric method for quantitation of heme using tetramethylbenzidine as substrate.. https://doi.org/10.1016/j.ab.2005.06.022

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PubMed2006

Electronic, infrared, and 1HNMR spectral studies of the novel charge-transfer complexes of o-tolidine and p-toluidine with alternation pi-acceptors (3,5-dinitro benzoic acid and 2,6-dichloroquinone-4-chloroimide) in CHCl3 solvent.

The rapid interaction between o-tolidine and p-toluidine (pi-donors) with the pi-acceptors, e.g., 3,5-dinitrobenzoic acid (DNB) and 2,6-dichloroquinone-4-chloroimide (DCQ) results in the formation of 1:1 charge-transfer complexes as the final products, [(o-tolidine) (acceptor)] and [(p-toluidine) (acceptor)]. The final products of the reactions have been isolated and characterized using FTIR, 1HNMR spectroscopy and elemental analysis as well as photometric titration. The stoichiometry and apparent formation constants of the complexes formed were determined by applying the conventional spectrophotometric molar ratio method.

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