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PubMed · 1609278

Triple helix-specific ligands.

J L Mergny·G Duval-Valentin·C H Nguyen·L Perrouault·B Faucon·M Rougée·T Montenay-Garestier·E Bisagni·C Hélène
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Abstract

A triple helix is formed upon binding of an oligodeoxynucleotide to the major groove of duplex DNA. A benzo[e]pyridoindole derivative (BePI) strongly stabilized this structure and showed preferential binding to a triplex rather than to a duplex. Energy transfer experiments suggest that BePI intercalates within the triple helix. Sequence-specific inhibition of transcription initiation of a specific gene by Escherichia coli RNA polymerase by a triplex-forming oligodeoxynucleotide is strongly enhanced when the triplex is stabilized by BePI. Upon irradiation with ultraviolet light, BePI induces covalent modifications of the target within the triple helix structure.

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Base SequenceCarbolinesDNADNA-Directed RNA PolymerasesEscherichia coliHot TemperatureIn Vitro TechniquesLigandsMolecular Sequence DataNucleic Acid HybridizationTranscription, GeneticJournal ArticleResearch Support, Non-U.S. Gov't

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J L Mergny, G Duval-Valentin, C H Nguyen, L Perrouault, B Faucon, M Rougée, T Montenay-Garestier, E Bisagni, C Hélène. 1992-06-19. Triple helix-specific ligands.. https://doi.org/10.1126/science.256.5064.1681

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