PubMed Health⌕ Search

PubMed · 16097704

[Optical detection system for micro biochemical analyses].

Abstract

For the need of biochemical chip, which consumes fewer specimens and is easy to integrate with micro-fluid chip, two kinds of spectrophotometric analysis methods are described in the present paper. Both the direct detection method and evanescent wave detection method are used in the experiments with visible light (460-800 nm). The experimental results proved that the direct detection is simple and evident; on the other hand the evanescent wave detection method consumes much less reagent and is easy to integrate with microchips.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Feng Li, Yi-hui Wu, Hua-bing Zhao, Hui Ju. 2005. [Optical detection system for micro biochemical analyses].. https://pubmed.ncbi.nlm.nih.gov/16097704/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

Nonlithographic fabrication of microfluidic devices.

A facile nonlithographic method for expedient fabrication of microfluidic devices of poly(dimethylsiloxane) is described. Positive-relief masters for the molds are directly printed on smooth substrates. For the formation of connecting channels and chambers inside the polymer components of the microfluidic devices, cavity-forming elements are adhered to the surfaces of the masters. Using this nonlithographic approach, we fabricated microfluidic devices for detection of bacterial spores on the basis of enhancement of the emission of terbium (III) ions.

Microfluidic Analytical Techniques↗

Reactions in droplets in microfluidic channels.

Fundamental and applied research in chemistry and biology benefits from opportunities provided by droplet-based microfluidic systems. These systems enable the miniaturization of reactions by compartmentalizing reactions in droplets of femoliter to microliter volumes. Compartmentalization in droplets provides rapid mixing of reagents, control of the timing of reactions on timescales from milliseconds to months, control of interfacial properties, and the ability to synthesize and transport solid reagents and products. Droplet-based microfluidics can help to enhance and accelerate chemical and biochemical screening, protein crystallization, enzymatic kinetics, and assays. Moreover, the control provided by droplets in microfluidic devices can lead to new scientific methods and insights.

Microfluidic Analytical Techniques↗

Periodic microfluidic bubbling oscillator: insight into the stability of two-phase microflows.

This Letter describes a periodically oscillating foam flow in a microfluidic device. For constant input parameters, both the produced bubble volume and the flow rate vary over a factor of two. We make explicit the link between the foam topology alternance and flow rate changes, and construct a retroaction model where bubbles still present downstream determine the volume of new bubbles, in agreement with experiment. This gives insight into the various parameters important to maintain monodispersity and at the same time shows a method to achieve controlled polydispersity.

Microfluidic Analytical Techniques↗