[Expression and characterization of the recombination anthrax lethal factor].

Qiang Guo; Jun-Jie Xu; Da-Yong Dong; Ling Fu; Wei Chen

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PubMed · 16110953

[Expression and characterization of the recombination anthrax lethal factor].

Qiang Guo·Jun-Jie Xu·Da-Yong Dong·Ling Fu·Wei Chen

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Abstract

The gene encoding anthrax lehtal factor (LF) was cloned into a secretory expression plasmid and then expressed in periplasmic space of E. coli. The recombinant LF (rLF) expressed was about 4% of the total proteins in E. coli. About 3 mg electrophoresis purity rLF could be obtained after the purification of 1 liter culure using ion exchange chromatography and gel filtration. The result of sequencing assay showed that the N-terminal amino acid sequence of rLF was identical to the N-terminal sequence of natural LF. In vitro toxicity analysis also shows that rLF has an excellent biological activity. The successful expression of rLF has placed a solid foundation for the research on toxicity mechanism of LF, developing new anthrax vaccines, and screening for inhibitors against anthrax toxin.

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Animals Antigens, Bacterial Bacterial Toxins Escherichia coli Mice Recombinant Proteins Journal Article Research Support, Non-U.S. Gov't

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Qiang Guo, Jun-Jie Xu, Da-Yong Dong, Ling Fu, Wei Chen. 2004. [Expression and characterization of the recombination anthrax lethal factor].. https://pubmed.ncbi.nlm.nih.gov/16110953/

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