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PubMed · 16114057

ELL binding regulates U19/Eaf2 intracellular localization, stability, and transactivation.

Wuhan Xiao·Feng Jiang·Zhou Wang
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Abstract

BACKGROUND: U19/Eaf2, an androgen-response gene, is downregulated in advanced human prostate cancer specimens and its overexpression can markedly induce apoptosis in prostate cancer cells. Eleven-nineteen Lysine-rich Leukemia (ELL) is an RNA polymerase II transcription elongation factor, initially identified as a fusion partner gene of MLL in the t(11; 19) (q23; p13.1) chromosomal translocation in acute myeloid leukemia. U19/Eaf2 was previously reported as an ELL-associated factor, a potential transcription factor binds to ELL, forming nuclear speckles in vivo. These findings suggest that ELL-U19/Eaf2 interaction is potentially important in prostate cancer progression and/or acute myeloid leukemia. However, the functional significance of U19/Eaf2 interaction with ELL remains unclear. METHODS: Using co-transfection, co-immunoprecipitation, protein stability assay and transactivation assay, we characterized the consequence of ELL binding to U19/Eaf2. RESULTS: We provide further evidence for U19/Eaf2 as a transcription factor and show that ELL binding is required for nuclear speckle formation of human U19/Eaf2, stabilizes U19/Eaf2 and enhances its transactivation activity. CONCLUSIONS: The above observations indicate ELL may be an important factor required for U19/Eaf2 function because U19/Eaf2 nuclear localization and transactivation activity are essential for its function as a transcription factor.

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Base SequenceBinding SitesCell Line, TumorGenes, ReporterHumansLuciferasesMaleMolecular Sequence DataOligodeoxyribonucleotidesPlasmidsProstatic NeoplasmsTranscription FactorsTranscriptional ActivationJournal ArticleResearch Support, N.I.H., Extramural

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Wuhan Xiao, Feng Jiang, Zhou Wang. 2006-01-01. ELL binding regulates U19/Eaf2 intracellular localization, stability, and transactivation.. https://doi.org/10.1002/pros.20309

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