Gene expression profiling in single cells within tissue.

Paola Capodieci; Michael Donovan; Heidi Buchinsky; Yusuf Jeffers; Carlos Cordon-Cardo; William Gerald; Jon Edelson; Shailesh M Shenoy; Robert H Singer

doi:10.1038/nmeth786

PubMed · 16118636

Gene expression profiling in single cells within tissue.

Paola Capodieci·Michael Donovan·Heidi Buchinsky·Yusuf Jeffers·Carlos Cordon-Cardo·William Gerald·Jon Edelson·Shailesh M Shenoy·Robert H Singer

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Abstract

We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.

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Biomarkers, Tumor Gene Expression Profiling Humans In Situ Hybridization, Fluorescence Male Neoplasm Proteins Paraffin Embedding Prostatic Neoplasms Tissue Distribution Tumor Cells, Cultured Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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Paola Capodieci, Michael Donovan, Heidi Buchinsky, Yusuf Jeffers, Carlos Cordon-Cardo, William Gerald, Jon Edelson, Shailesh M Shenoy, Robert H Singer. 2005. Gene expression profiling in single cells within tissue.. https://doi.org/10.1038/nmeth786

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