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Spectral-domain optical coherence phase microscopy for quantitative phase-contrast imaging.

Chulmin Joo·Taner Akkin·Barry Cense·Boris H Park·Johannes F de Boer
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Abstract

We describe a novel microscopy technique for quantitative phase-contrast imaging of a transparent specimen. The technique is based on depth-resolved phase information provided by common path spectral-domain optical coherence tomography and can measure minute phase variations caused by changes in refractive index and thickness inside the specimen. We demonstrate subnanometer level path-length sensitivity and present images obtained on reflection from a known phase object and human epithelial cheek cells.

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Cells, CulturedEpithelial CellsEquipment DesignEquipment Failure AnalysisHumansImage EnhancementImaging, Three-DimensionalMicroscopy, Phase-ContrastReproducibility of ResultsSensitivity and SpecificitySpectrum AnalysisTomography, Optical CoherenceEvaluation StudyJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, U.S. Gov't, P.H.S.

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Chulmin Joo, Taner Akkin, Barry Cense, Boris H Park, Johannes F de Boer. 2005-08-15. Spectral-domain optical coherence phase microscopy for quantitative phase-contrast imaging.. https://doi.org/10.1364/ol.30.002131

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