Variation in S phase in synchronous human cell lines.

L N Kapp; A J Millis; D A Pious

doi:10.1007/bf02618245

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Variation in S phase in synchronous human cell lines.

L N Kapp·A J Millis·D A Pious

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Abstract

Growth parameters of diploid and trisomic human fibroblasts were determined. The rate of growth of both classes of cells was examined in asynchronous cultures, and diploid and trisomic cells had similar growth rates. Synchronous cultures were developed using simple mitotic selection. The patterns and length of the DNA synthetic period (S phase) were found to be altered in trisomy 21 cells when compared to diploid human or to heteroploid HeLa cells. Early S-phase synthesis was absent or reduced and the overall length of the S phase was extended. However, the trisomic cells have apparently normal rates of DNA chain elongation and normal replicon sizes.

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Cell Cycle Cell Division Cell Line DNA DNA Replication DNA, Neoplasm Diploidy Down Syndrome Fibroblasts HeLa Cells Humans Interphase Time Factors Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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L N Kapp, A J Millis, D A Pious. 1979. Variation in S phase in synchronous human cell lines.. https://doi.org/10.1007/bf02618245

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