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PubMed · 16128989

Androgens induce CD-9 in human prostate tissue.

Y Chuan·S-T Pang·A Bergh·G Norstedt·A Pousette
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Abstract

Based on microarray analyses of LNCaP and LNCaP-r prostatic cell-lines we tentatively identified CD-9 as an androgen sensitive protein. This prompted us to characterize the androgen sensitivity and the correlation to malignancy of CD-9 at the protein level. Using Western blot, RT-PCR and immunohistochemistry the expression of CD-9 was analysed in LNCaP cells stimulated during increasing time by the synthetic androgen R1881 and also in 88 specimens of human prostate cancer tissues. Expression of CD-9 was induced by R1881 in LNCaP. CD-9 was immunolocalized in human prostate tissue sections representing non-malignant tissue as well as tumour areas. In non-malignant glands CD-9 immunoreactivity was observed at the apical and lateral cell borders of luminal epithelial cells. Basal epithelial cells were largely unstained. In tumour areas CD-9 staining intensity was variable and apparently not related to primary Gleason grade. In prostate tissue from a patient under androgen ablation therapy no staining was observed in luminal epithelial cells or in the tumour areas, but some staining was observed in basal epithelial cells. CD-9 is regulated by androgens in LNCaP and present in human prostate specimens. The expression is variable and changes in expression levels. These and earlier studies using other tissues indicate that CD-9 and its cellular localization could have an important role in prostate cancer cell development.

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AndrogensAntigens, CDBlotting, WesternCell Line, TumorGene Expression RegulationHumansMaleMembrane GlycoproteinsMetriboloneProstateProstatic NeoplasmsReverse Transcriptase Polymerase Chain ReactionTetraspanin 29Journal ArticleResearch Support, Non-U.S. Gov't

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Y Chuan, S-T Pang, A Bergh, G Norstedt, A Pousette. 2005. Androgens induce CD-9 in human prostate tissue.. https://doi.org/10.1111/j.1365-2605.2005.00552.x

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