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PubMed · 1613045

Surface-modified membranes as a matrix for protein purification.

P Langlotz·K H Kroner
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Abstract

Recently introduced membrane-based chromatographic supports for protein separation are available either with a coupled ligand, e.g., protein A, protein G or ion-exchange groups, or as activated matrices for coupling a desired ligand. The coupling conditions for protein A and immunoglobulin G to an epoxy-activated membrane were determined. The performance of the prepared affinity membranes was investigated using pure rabbit immunoglobulin G and protein A as a model system. For practical application monoclonal antibodies from cell culture supernatant were purified with a prepared protein A membrane and for comparison with a sulphonic acid ion exchange membrane.

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Antibodies, MonoclonalChromatography, AffinityChromatography, Ion ExchangeElectrophoresis, Polyacrylamide GelImmunoglobulin GMembranes, ArtificialProteinsStaphylococcal Protein AJournal ArticleResearch Support, Non-U.S. Gov't

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P Langlotz, K H Kroner. 1992-02-07. Surface-modified membranes as a matrix for protein purification.. https://doi.org/10.1016/0021-9673(92)80227-l

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