Characterization of lipids in complex samples using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry.

Eric Jover; Mohamed Adahchour; Josep M Bayona; René J J Vreuls; Udo A Th Brinkman

doi:10.1016/j.chroma.2005.05.093

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Characterization of lipids in complex samples using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry.

Eric Jover·Mohamed Adahchour·Josep M Bayona·René J J Vreuls·Udo A Th Brinkman

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Abstract

Most lipids are a complex mixture of classes of compounds such as fatty acids, fatty alcohols, diols, sterols and hydroxy acids. In this study, the suitability of comprehensive two-dimensional gas chromatography coupled to a time-of-light mass spectrometer is studied for lipid characterization in complex samples. With lanolin, a refined wool wax, as test sample, it is demonstrated that combined methylation plus silylation is the preferred derivatization procedure to achieve (i) high-quality GC x GC separation and (ii) easily recognizable ordered structures in lipid analysis. Optimization of the GC x GC column combination, the influence of the temperature programme on the quality of the separation, and the potential and limitations of automated TOF-MS-based identification are discussed. The combined power of a 2D separation, ordered structures and MS detection is illustrated by the identification of several minor sample constituents.

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Eric Jover, Mohamed Adahchour, Josep M Bayona, René J J Vreuls, Udo A Th Brinkman. 2005-09-09. Characterization of lipids in complex samples using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry.. https://doi.org/10.1016/j.chroma.2005.05.093

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