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Chitinase production in solid-state fermentation by Enterobacter sp. NRG4 using statistical experimental design.

Neetu Dahiya·Rupinder Tewari·Ram Prakash Tiwari·Gurinder Singh Hoondal
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Abstract

The optimization of nutrient levels for chitinase production by Enterobacter sp. NRG4 in solid-state fermentation conditions (SSF) was carried out using response surface methodology (RSM) based on central composite design (CCD). The design was employed by selecting wheat bran-to-flake chitin ratio, moisture level, inoculum size, and incubation time as model factors. The results of first-order factorial design experiments showed that all four independent variables have significant effects on chitinase production. The optimum concentrations for chitinase production were wheat bran-to-flake chitin ratio, 1; moisture level, 80%; inoculum size, 2.6 mL; and incubation time, 168 h. Using this statistical optimization method, chitinase production was found to increase from 616 U . g(-1) dry weight of solid substrate to 1475 U . g(-1) dry weight of solid substrate.

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ChitinChitinasesEnterobacterFermentationResearch DesignSubstrate SpecificityTemperatureJournal ArticleResearch Support, Non-U.S. Gov't

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Neetu Dahiya, Rupinder Tewari, Ram Prakash Tiwari, Gurinder Singh Hoondal. 2005-08-24. Chitinase production in solid-state fermentation by Enterobacter sp. NRG4 using statistical experimental design.. https://doi.org/10.1007/s00284-005-4520-y

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